Rapid Diagnosis of MEN2B Using Unlabeled Probe Melting Analysis and the LightCycler 480 Instrument
نویسندگان
چکیده
منابع مشابه
Rapid High-Throughput Methylation Analysis Using the LightCycler® 480 System
Michael Krypuy1, Tomasz K. Wojdacz2, and Alexander Dobrovic1,3 1Molecular Pathology Research and Development Laboratory, Department of Pathology, Peter MacCallum Cancer Centre, Melbourne, Australia; 2Institute of Human Genetics, University of Aarhus, Aarhus, Denmark; 3Department of Pathology, University of Melbourne, Parkville, Australia *Corresponding authors: [email protected], ...
متن کاملSNP genotyping by unlabeled probe melting analysis.
Fluorescent nucleic acid detection in polymerase chain reaction (PCR) generally uses oligonucleotide probes labeled with covalently attached dyes. However, unlabeled oligonucleotides in the presence of saturating DNA dyes can also serve as hybridization probes. The DNA dye, LCGreen Plus, and a 3'-blocked unlabeled probe are added before amplification, and asymmetric PCR is performed at a 1:5 to...
متن کاملHigh-resolution melting curve analysis on the LightCycler® 480 PCR system
480 PCR system Roche Applied Science ́s LightCycler® family of real-time PCR systems offer fast, accurate and versatile platforms for genetic variation research. The new plate-based LightCycler® 480 System provides the temperature homogeneity and optical characteristics required for high-performance melting-curve analysis (MCA). On the level of data acquisition and available detection channels, ...
متن کاملthe analysis of the role of the speech acts theory in translating and dubbing hollywood films
از محوری ترین اثراتی که یک فیلم سینمایی ایجاد می کند دیالوگ هایی است که هنرپیش گان فیلم میگویند. به زعم یک فیلم ساز, یک شیوه متأثر نمودن مخاطب از اثر منظوره نیروی گفتارهای گوینده, مثل نیروی عاطفی, ترس آور, غم انگیز, هیجان انگیز و غیره, است. این مطالعه به بررسی این مسأله مبادرت کرده است که آیا نیروی فراگفتاری هنرپیش گان به مثابه ی اعمال گفتاری در پنج فیلم هالیوودی در نسخه های دوبله شده باز تولید...
15 صفحه اولInfluence of DNA polymerases on quantitative PCR results using TaqMan probe format in the LightCycler instrument.
Real-time fluorescence polymerase chain reaction (PCR) techniques are increasingly used to quantitate target sequences for diagnostic and research purposes. Currently, the so called TaqMan probe chemistry is mostly used as fluorogenic system. This probe format is strictly dependent on the 5'-exonuclease activity of DNA polymerase as fragmentation of the probe during the reaction is essential fo...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Journal of Molecular Diagnostics
سال: 2008
ISSN: 1525-1578
DOI: 10.2353/jmoldx.2008.070111